Part:BBa_K4630102

stgRNA-barcode-cassette (1+2)

This part is the combination of the stgRNA-barcode-cassette 1 (BBa_K4630100) and stgRNA-barcode-cassette 2 (BBa_K4630101). With the two cassettes, it can record the triggering event twice.

The principle of its recording is that when the system responds to the first signal and the first level knockout occurs, the terminator before the second level stgRNA2 disappears. Therefore, when the system receives the second signal stimulation, it can activate the expression of secondary stgRNA2, targeting self-knockout. Thus achieving multi-level recording. The entire system presents an unlocking model. The terminator in the first level sequence is the lock of the second level sequence. Only when the first level performs self-knocking can the lock of the second level be opened, expressing the second level normally.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 55
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 94